WebbPCR amplification: DNA polymerase: 2× KAPA HiFi HotStart ReadyMix: Phusion Green Hot Start II High-Fidelity Master Mix: Phire Hot Start II DNA Polymerase: PCRBIO HIFI polymerase: PCR cycling conditions: 95 °C 3 min, 35× (98 °C 30 s, 57 °C 30 s, 72 °C 30 s), 72 °C 1 min: 98 °C 2 min, 30× (98 °C 15 s, 52 °C 30 s, 72 °C 30 s), 72 °C 3 min Webb2X Phire Plant Direct PCR Master Mix has been optimized for Direct PCR from plant material. It contains the dNTPs and provides 1.5 mM MgCl 2 concentration in the final reaction. It also includes a density reagent and two tracking dyes for direct loading of PCR product on a gel. The Master Mix employes Phire Hot Start II DNA Polymerase, that ...
Thermo Scientific PCR Reagents Selection Guide
WebbHot Start PCR Reagents and Kits Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl 2 Chemically modified form of AmpliTaq DNA Polymerase … WebbPhire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as … porsche 911 rsr wallpaper 4k
Hot Start PCR Reagents and Kits Fisher Scientific
WebbThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. Webb1 aug. 2024 · RT-PCR was performed with the Phire Green Hot Start II PCR Master Mix (Thermo Scientific) and primers in Table 2. EB formation hiPSCs were enzimatically detached and grown for 7 days in non-adherent conditions in a modified iPS medium deprived of bFGF and containing 20% FBS instead of KO-SR. WebbThermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA … porsche 911 rsr sound mod